藏茵陈HPLC指纹图谱及模式识别研究

田成旺,张铁军,蒋伶活

中国药学杂志 ›› 2013, Vol. 48 ›› Issue (18) : 1545-1549.

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中国药学杂志 ›› 2013, Vol. 48 ›› Issue (18) : 1545-1549. DOI: 10.11669/cpj.2013.18.007
论著

藏茵陈HPLC指纹图谱及模式识别研究

  • 田成旺12,张铁军2,蒋伶活13
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Study on Swertia mussotii Franch. by HPLC Fingerprinting Method Combined with Chemical Pattern Recognition Approaches

  • TIAN Cheng-wang1,2, ZHANG Tie-jun2,JIANG Ling-huo1,3
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摘要

目的研究藏茵陈的质量控制方法。方法采用高效液相色谱法建立藏茵陈药材的指纹图谱,收集了19批样品进行测定,并使用主成分分析对指纹图谱进行了模式识别研究,采用HPLC-MS技术对色谱峰进行了初步归属。结果对11个主要色谱峰进行了指认,通过主成分分析可知,獐芽菜苦苷和当药醇苷为藏茵陈质量控制中比较重要的指标。结论本方法为藏茵陈药材的质量综合评价和资源合理利用奠定了基础。

Abstract

OBJECTIVE To study the quality control method of Swertia mussotii Franch. METHODS A high performance liquid chromatographic method was developed to establish the fingerprint of Swertia mussotii Franch. Nineteen samples from various batches were analyzed, furthermore, PCA were used to differentiate and evaluate the whole fingerprints. Some characteristic peaks were identified preliminarily with HPLC-MS method. RESULTS Eleven peaks were identified by MS. Swertiamarin and swertianolin were the key components for quality control. CONCLUSION The method provides an academic reference for controlling the quality of Swertia mussotii Franch.

关键词

川西獐牙菜 / 指纹图谱 / 主成分分析 / 液质联用技术

Key words

Swertia mussotii Franch. / fingerprint / principal component analysis / HPLC-MS

引用本文

导出引用
田成旺,张铁军,蒋伶活. 藏茵陈HPLC指纹图谱及模式识别研究[J]. 中国药学杂志, 2013, 48(18): 1545-1549 https://doi.org/10.11669/cpj.2013.18.007
TIAN Cheng-wang,, ZHANG Tie-jun,JIANG Ling-huo,. Study on Swertia mussotii Franch. by HPLC Fingerprinting Method Combined with Chemical Pattern Recognition Approaches[J]. Chinese Pharmaceutical Journal, 2013, 48(18): 1545-1549 https://doi.org/10.11669/cpj.2013.18.007
中图分类号: R284   

参考文献

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基金

国家自然科学基金资助项目(81001627);天津市自然科学基金资助项目(10JCZDJC21400)
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