目的研究藏茵陈的质量控制方法。方法采用高效液相色谱法建立藏茵陈药材的指纹图谱，收集了19批样品进行测定，并使用主成分分析对指纹图谱进行了模式识别研究，采用HPLC-MS技术对色谱峰进行了初步归属。结果对11个主要色谱峰进行了指认，通过主成分分析可知，獐芽菜苦苷和当药醇苷为藏茵陈质量控制中比较重要的指标。结论本方法为藏茵陈药材的质量综合评价和资源合理利用奠定了基础。
Abstract
OBJECTIVE To study the quality control method of Swertia mussotii Franch. METHODS A high performance liquid chromatographic method was developed to establish the fingerprint of Swertia mussotii Franch. Nineteen samples from various batches were analyzed, furthermore, PCA were used to differentiate and evaluate the whole fingerprints. Some characteristic peaks were identified preliminarily with HPLC-MS method. RESULTS Eleven peaks were identified by MS. Swertiamarin and swertianolin were the key components for quality control. CONCLUSION The method provides an academic reference for controlling the quality of Swertia mussotii Franch.
关键词
川西獐牙菜 /
指纹图谱 /
主成分分析 /
液质联用技术
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Key words
Swertia mussotii Franch. /
fingerprint /
principal component analysis /
HPLC-MS
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中图分类号：
R284
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参考文献
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脚注
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基金
国家自然科学基金资助项目(81001627)；天津市自然科学基金资助项目(10JCZDJC21400)
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